Polypyrrole-coated poly(l-lactic acid-co-ε-caprolactone)/silk fibroin nanofibrous membranes promoting neural cell proliferation and differentiation with electrical stimulation

• DOI: 10.1039/c6tb01710j
• Publication Date: 2016-09-22T20:01:50Z
• Authors: Binbin Sun; Tong Wu; Juan Wang; Dawei Li; Jing Wang; Qiang Gao; M. Aqeel Bhutto; Hany El-Hamshary; Salem S. Al-Deyab; Xiumei Mo
• Keywords: Fibroin; Polypyrrole; Electrospinning; Thermogravimetric analysis; Caprolactone

Polypyrrole (Ppy), as a conductive polymer, is commonly used for nerve tissue engineering because of its good conductivity and non-cytotoxicity. To avoid the inconvenience Ppy processing, it was coated on electrospun poly(l-lactic acid-co-ε-caprolactone)/silk fibroin (PLCL/SF) nanofibers via in situ oxidative polymerization pyrrole monomers this study. Ppy-coated PLCL/SF membranes were characterized by scanning electron microscopy (SEM), atomic force (AFM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric (TG) analysis. The results confirmed disposition nanofibers, kept their nanofibrous morphology thermal stability, comparison to untreated ones. conductivities water contact angles evaluated well, indicated that hydrophilicity increased. Furthermore, study showed electrical stimulation (ES) promoted PC12 cell differentiation axonal extension nanofibers. MTT assay suggested both ES could promote Schwann (SC) proliferation. Immunofluorescence staining real time-qPCR (RT-qPCR) testing demonstrated induce even without growth factor (NGF) treatment, moreover, coating increased inducing effects differentiation. overall promising potential peripheral repair regeneration.