Efficient delivery of chlorin e6 into ovarian cancer cells with octalysine conjugated superparamagnetic iron oxide nanoparticles for effective photodynamic therapy

• DOI: 10.1039/c6tb01988a
• Publication Date: 2016-11-07T19:53:29Z
• Authors: Li Zhao; Hongkuan Yang; Tsukuru Amano; Hongmei Qin; Luyi Zheng; Akimasa Takahashi; Shiguang Zhao; Ikuo Tooyama; Takashi Murakami; Naoki Komatsu
• Keywords: Chlorin; Superparamagnetism

In cancer treatment, efficient delivery of active anticancer drugs into cells is highly desirable for maximizing therapeutic effects and alleviating side effects. this work, a nanocarrier consisting an Fe3O4 core, polyglycerol coating, octalysine functionality (SPION-PG-Lys8) has been designed, synthesized used to deliver photosensitizer, chlorin e6 (Ce6), photodynamic therapy (PDT) cells. SPION-PG-Lys8 colloidally stable in various aqueous solutions, showing high positive zeta potential 47.2 ± 6.9 mV pure water. vitro characterization reveals that efficiently taken up by SKOV3 ovarian cells, exhibiting low cytotoxicity, suppressed autophagy compared bare SPIONs. Negatively charged Ce6 thus loaded on the through electrostatic attraction yield SPION-PG-Lys8/Ce6 nanocomplex with 22.4 4.3 mV. more easily than free Ce6, surprisingly, internalized found be enriched mitochondria. exhibits almost no cytotoxicity under dark conditions, but strong photocytotoxicity due light-triggered production reactive oxygen species (ROS) destroying Taken together, our results highlight great as carrier therapy.