A co-expression strategy to achieve labeling of individual subunits within a dimeric protein for single molecule analysis

• DOI: 10.1039/c7cc03032k
• Publication Date: 2017-06-19T14:33:51Z
• Authors: Fei Lou; Jie Yang; Si Wu; Sarah Perrett
• Keywords: Models, Molecular; 0301 basic medicine; Glutathione Peroxidase; 03 medical and health sciences; Saccharomyces cerevisiae Proteins; Prions; Fluorescence Resonance Energy Transfer; Gene Expression Regulation, Bacterial; Saccharomyces cerevisiae; Protein Multimerization; Single Molecule Imaging

Site-specific incorporation of donor–acceptor pair into a dimeric protein for single-molecule FRET analysis is realized based on a co-expression strategy.