pH-Mediated molecular differentiation for fluorimetric quantification of chemotherapeutic drugs in human plasma

• DOI: 10.1039/c7cc07668a
• Publication Date: 2018-01-23T09:24:01Z
• Authors: Luis A. Serrano; Ye Yang; Elisa Salvati; Francesco Stellacci; Silke Krol; Stefan Guldin
• Keywords: Chemotherapeutic drugs

At present, drug dosage is based on standardised approaches that disregard pharmakokinetic differences between patients and lead to non-optimal efficacy unnecessary side effects. In this work, we demonstrate the potential of pH-mediated fluorescence spectroscopy for therapeutic monitoring in complex media. We apply principle simultaneous quantification chemotherapeutic prodrug Irinotecan its active metabolite SN-38 from human plasma across clinically relevant concentration range, i.e. micromolar nanomolar at molar ratios up 30 : 1.