Effects of ambient PM2.5and 9-nitroanthracene on DNA damage and repair, oxidative stress and metabolic enzymes in the lungs of rats

• DOI: 10.1039/c7tx00065k
• Publication Date: 2017-05-18T08:54:02Z
• Authors: Ruijin Li; Lifang Zhao; Li Zhang; Minghui Chen; Jing Shi; Chuan Dong; Zongwei Cai
• Keywords: XRCC1; Malondialdehyde

Ambient fine particulate matter (PM2.5) is a complex mixture associated with lung cancer risk. PM2.5-bound nitro-polycyclic aromatic hydrocarbons (NPAHs) have been demonstrated to possess mutagenicity and carcinogenicity. Previous studies showed that PM2.5 induced DNA damage, whereas there little knowledge of whether 9-nitroanthracene (9-NA), typical compound NPAHs in PM2.5, causes damage. Also, the regulating mechanisms 9-NA damage repair are not yet fully established. Here we sought investigate molecular lungs male Wistar rats exposed (1.5 mg per kg body weight) or three different dosages 9-NA. And then strand breaks, 8-OH-dG formation, DNA-protein crosslink gene expressions rat were analyzed. In addition, alteration oxidative stress factors metabolic enzymes detected. The results (1) higher dosage (4.0 × 10-5 1.2 10-4 significantly caused accompanied by increasing OGG1 expression while inhibiting MTH1 XRCC1 expression, elevating levels GADD153, hemeoxygenase-1 malondialdehyde, promoting activities CYP450 isozymes glutathione S-transferase. (2) 1.3 kg-1 exposure couldn't cause stress. (3) At approximately equivalent dose level, PM2.5-induced effects more obvious than positive correlation. It suggests may be mediated partially through influencing capacity enhancing biotransformation, this negative effect might related genotoxicity.