Does conjugation strategy matter? Cetuximab-conjugated gold nanocages for targeting triple-negative breast cancer cells

• DOI: 10.1039/c9na00241c
• Publication Date: 2019-07-23T07:05:00Z
• Authors: S. Avvakumova; L. Pandolfi; E. Soprano; L. Moretto; M. Bellini; E. Galbiati; M. A. Rizzuto; M. Colombo; R. Allevi; F. Corsi; A. Sánchez Iglesias; D. Prosperi
• Keywords: Nanocages; Internalization; Bioconjugation; Triple-negative breast cancer; Rational design

The efficient targeting of cancer cells depends on the success obtaining active overexpressed receptors. A very accurate design nanoconjugates should be done via selection conjugation strategy to achieve effective targeted nanoconjugates. Here, we present a detailed study cetuximab-conjugated nonspherical gold nanocages for triple-negative breast cells, including MDA-MB-231 and MDA-MB-468. few different general strategies were selected monoclonal antibody nanoparticle surface. By varying bioconjugation conditions, orientation or presence polymeric spacer recombinant protein biolinker, demonstrate importance rational quantitative content ICP-AES allowed us compare effectiveness cellular uptake as function confirmed nature internalization in epidermal growth factor receptor recognition, corroborating nanomaterials nanomedicine.