An integrated microfluidic platform for selective and real-time detection of thrombin biomarkers using a graphene FET

• DOI: 10.1039/d0an00251h
• Publication Date: 2020-05-06T17:03:33Z
• Authors: Niazul I. Khan; Mohammad Mousazad...; Sujoy Ghosh; John G. Tsavalas; Edward Song
• Keywords: Aptamer; Lab-on-a-Chip; Point of care

Lab-on-a-chip technology offers an ideal platform for low-cost, reliable, and easy-to-use diagnostics of key biomarkers needed early screening diseases other health concerns. In this work, a graphene field-effect transistor (GFET) functionalized with target-binding aptamers is used as biosensor the detection thrombin protein biomarker. Furthermore, GFET integrated microfluidic device enhanced sensing performances in terms limit, sensitivity, continuous monitoring. Under platform, picomolar limit was achieved measuring thrombin; our experiment measured low 2.6 pM. FTIR, Raman UV-Vis spectroscopy measurements were performed to confirm functionalization steps. Based on concentration-dependent calibration curve, dissociation constant KD = 375.8 pM obtained. Continuous real-time also conducted under gate voltage (VGS) observe transient response sensor when analyte introduced device. The target selectivity evaluated confirmed by challenging various concentrations lysozyme protein. results suggest that has potential be general diagnostic clinically relevant point-of-care applications.