Real-time isothermal DNA amplification monitoring in picoliter volumes using an optical fiber sensor

• DOI: 10.1039/d0lc01069c
• Publication Date: 2020-11-26T12:44:50Z
• Authors: Monika Janik; Seyed Vahid Hamidi; Marcin Koba; Jonathan Perreault; Ryan Walsh; Wojtek J. Bock; Mateusz Śmietana
• Keywords: Influenza A Virus, H5N1 Subtype; -; Biosensing Techniques; DNA; Nucleic Acid Amplification Techniques; 01 natural sciences; Optical Fibers; 620; 0104 chemical sciences; 3. Good health

Rolling circle amplification (RCA) of DNA can be considered as a great alternative to the gold standard polymerase chain reaction (PCR), where rapid, sensitive, and reliable test results are required.