Efficient biodegradation of malachite green by an artificial enzyme designed in myoglobin

• DOI: 10.1039/d1ra02202d
• Publication Date: 2021-04-30T08:34:22Z
• Authors: Heng-Fang Xiang; Jia-Kun Xu; Jiao Liu; Xin-Zhi Yang; Shu-Qin Gao; Ge-Bo Wen; Ying-Wu Lin
• Keywords: Malachite green

Synthetic dyes such as malachite green (MG) have a wide range of applications. Meanwhile, they bring great challenges for environmental security and cause potential damages to human health. Compared with traditional approaches, enzymatic catalysis is an emerging technique wastewater treatment. As alternatives natural enzymes, artificial enzymes received much attention In previous studies, we rationally designed based on myoglobin (Mb), by introducing distal histidine (F43H mutation) creating channel the heme pocket (H64A mutation). We herein show that enzyme F43H/H64A Mb can be successfully applied efficient biodegradation MG under weak acid conditions. The degradation efficiency higher than those dye-decolorizing peroxidase laccase (13-18-fold). interaction was investigated using both experimental molecular docking products were also revealed UPLC-ESI-MS analysis. Based these results, proposed plausible mechanism MG. With high-yield overexpression in E. coli cells, this study suggests has applications fisheries textile industries.