Chinese chestnut shell polyphenol extract regulates the JAK2/STAT3 pathway to alleviate high-fat diet-induced, leptin-resistant obesity in mice

• DOI: 10.1039/d3fo00604b
• Publication Date: 2023-04-26T09:20:51Z
• Authors: Suwen Liu; Wenhong Jiang; Chang Liu; Shuo Guo; Hao Wang; Xuedong Chang
• Keywords: Fat accumulation

Chinese chestnut shell is a by-product of food processing and rich in polyphenols. This study sought to investigate the effect polyphenol extract (CSP) on weight loss lipid reduction 12-week high-fat diet (HFD)-induced murine obesity model. CSP (300 mg per kg body weight) was administered intragastrically daily. AG490, JAK2 protein tyrosine kinase inhibitor, also intraperitoneally injected. The results showed that an HFD induced leptin resistance (LR). Compared corresponding values group, treatment improved blood levels, weight, levels obese mice (p < 0.01). Additionally, enhanced enzyme activity by improving total antioxidant capacity, attenuating oxidative stress, reducing fat droplet accumulation inflammation liver, epididymal, retroperitoneal adipose tissue. activated LEPR-JAK2/STAT3-PTP1B-SOCS-3 signal transduction pathway hypothalamus tissue LR while regulating expression proteins related metabolism (PPARγ, FAS, LPL) white cavity. However, amelioration dependent JAK2. Molecular docking simulation further demonstrated strong binding affinity procyanidin C1 (-10.3983297 kcal mol-1) B1 (-9.12686729 crystal structure These suggest may be used reduce HFD-induced with potential application as functional additive.