Simultaneous detection of 5-methylcytosine and 5-hydroxymethylcytosine at specific genomic loci by engineered deaminase-assisted sequencing

• DOI: 10.1039/d4sc00930d
• Publication Date: 2024-05-20T09:40:39Z
• Authors: Neng-Bin Xie; Min Wang; Tong-Tong Ji; Xia Guo; Fang-Yin Gang; Ying Hao; Li Zeng; Ya-Fen Wang; Yu-Qi Feng; Bi-Feng Yuan
• Keywords: 0301 basic medicine; Chemistry; 03 medical and health sciences

We developed the EDA-seq method, which enables the simultaneous and quantitative detection of C, 5mC, and 5hmC in DNA at single-base resolution.