Modular access to nucleobase GFP-surrogates: pH-responsive smart probes for ratiometric nucleic acid diagnostics

• DOI: 10.1039/d4sc07994a
• Publication Date: 2025-03-13T15:41:00Z
• Authors: Keenan T. Regan; Austin Pounder; Ryan E. Johnson; Makay T. Murray; Hannah X. Glowacki; Stacey D. Wetmore; Richard A. Mander...

Aldol condensation between phenolic aldehydes and a latent enolate indanone handle within DNA oligonucleotides provides access to fluorescent nucleobase GFP-surrogates with smart features for ratiometric detection strategies.