Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells

• DOI: 10.1042/bcj20170891
• Publication Date: 2018-04-18T15:02:40Z
• Authors: Virginia Actis Dato; Rubén A. Grosso; María C. Sánchez; Claudio M. Fader; Gustavo A. Chiabr...
• Keywords: LRP1; Rab; GLUT4

Low-density lipoprotein (LDL) receptor-related protein-1 (LRP1) is expressed in retinal Müller glial cells (MGCs) and regulates intracellular translocation to the plasma membrane (PM) of proteins involved cellular motility activity. Different functions MGCs may be influenced by insulin, including removal extracellular glutamate retina. In present work, we investigated whether insulin promotes LRP1 PM glial-derived cell line MIO-M1 (human cell-derived line). We demonstrated that stored small vesicles containing an approximate size 100 nm (mean diameter range 100-120 nm), which were positive for sortilin VAMP2, also incorporated GLUT4 when it was transiently transfected. Next, observed promoted insulin-regulated exocytosis through activation IR/PI3K/Akt axis Rab-GTPase such as Rab8A Rab10. addition, these Rab-GTPases regulated both constitutive insulin-induced PM. Finally, found dominant-negative Rab10 mutants impaired signaling axis, suggesting GTPase well level at surface are IR activation.