Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression

• DOI: 10.1042/bj20071474
• Publication Date: 2008-03-14T17:04:46Z
• Authors: Constance Delaby; Nathalie Pilard; Hervé Puy; François Canonne-...
• Keywords: Ferroportin; Catabolism; Transcription

Tissue macrophages play an essential role in iron recycling through the phagocytosis of senescent RBCs (red blood cells). Following haem catabolism by HO1 (haem oxygenase 1), they recycle back into plasma exporter Fpn (ferroportin). We previously described a cellular model EP (erythrophagocytosis), based on primary cultures mouse BMDMs (bone-marrow-derived macrophages) and aged murine RBCs, showed that induces changes expression profiles HO1. In present paper, we demonstrate derived from human or exogenous source led to marked transcriptional activation genes. Iron released subsequently stimulated mRNA protein associated with localization transporter at cell surface, which probably promotes export plasma. These findings highlight dual mechanism regulation BMDMs, characterized early induction gene transcription predominantly mediated haem, followed iron-mediated post-transcriptional exporter.