The distribution and expression of a biotrophy‐related gene,CIH1, within the genusColletotrichum

• DOI: 10.1046/j.1364-3703.2000.00025.x
• Publication Date: 2003-03-12T14:37:24Z
• Authors: Sarah E. Perfect; Katherine L. Pixton; Richard J. O’Connell; Jonathan R. Green
• Keywords: Colletotrichum lindemuthianum; Sclerotinia Sclerotiorum; Colletotrichum

Abstract During the biotrophic phase of infection process hemibiotrophic anthracnose fungus Colletotrichum lindemuthianum, an intracellular hypha develops within epidermal cells its host, Phaseolus vulgaris. This is followed by formation secondary hyphae during necrotrophic phase. Previous work using a monoclonal antibody, UB25, has identified glycoprotein that specific to interfacial matrix forms between wall and invaginated host plasma membrane. The gene encoding protein UB25 was cloned immunoscreening designated CIH1. predicted amino acid sequence revealed proline-rich glycoprotein, biochemical evidence suggested it formed cross-linked structure at interface. Although CIH1 fungal gene, product several similarities plant cell proteins. In this paper, we have surveyed distribution expression genus Colletotrichum, encompassing both species. results show homologues are present in all species tested. Northern blot studies time course planta shown expressed C. lindemuthianum bean trifolii alfalfa development. Immunofluorescence labelling infected strips with destructivum as infects were specifically labelled similar way those bean. Western analysis showed also vitro, though not constitutively. Overall, supports role for biotrophy Colletotrichum.