Placenta growth factor (PlGF) induces vascular endothelial growth factor (VEGF) secretion from mononuclear cells and is co-expressed with VEGF in synovial fluid

• DOI: 10.1046/j.1365-2249.2000.01097.x
• Publication Date: 2003-03-11T10:19:33Z
• Authors: M J Bottomley; N J A Webb; C J Watson; L Holt; M Bukhari; J Denton; A J Freemont; P E C Brenchley
• Keywords: Adult; Aged, 80 and over; Male; Lymphokines; 0303 health sciences; Adolescent; Arthritis; Endothelial Growth Factors; In Vitro Techniques; Middle Aged; Pregnancy Proteins; 3. Good health; Leukocyte Count; 03 medical and health sciences; Case-Control Studies; Synovial Fluid; Leukocytes, Mononuclear; Humans; Female; Joint Diseases; Growth Substances; Aged; Placenta Growth Factor

SUMMARY The aims of this study were (i) to determine whether PlGF, VEGF and PlGF/VEGF heterodimers are detected in synovial fluid (SF) plasma samples from patients with a range arthropathies; (ii) describe any correlation exists between SF heterodimer levels the total differential leucocyte counts; (iii) investigate regulation peripheral blood mononuclear cell (PBMC) secretion by stimuli relevant inflammatory joints. measured arthropathies normal controls ELISA. Western blotting for PlGF was performed on three rheumatoid arthritis (RA) primary arthropathies. quantified culture supernatants after stimulation lipopolysaccharide (LPS), or cobalt ions PBMC isolated RA controls. all samples. 10.2% samples, most frequently confirmed presence SF. 52% 31% control 38% 21% none In arthropathy patients, correlated significantly count neutrophil count. potent inducer production both subjects. This is first report detection arthropathies, we have shown time that up-regulates production. may therefore play key role joint.