Extensive trans-species mitochondrial polymorphisms in the carabid beetles Carabus subgenus Ohomopterus caused by repeated introgressive hybridization

• DOI: 10.1046/j.1365-294x.2001.t01-1-01404.x
• Publication Date: 2003-03-12T10:41:45Z
• Authors: T. Sota; R. Ishikawa; M. Ujiie; F. Kusumoto; A. P. Vogler
• Keywords: Introgression; Subgenus; Subspecies; Monophyly; Reproductive isolation; Polyphyly; Lineage (genetic)

To study the potential importance of introgressive hybridization to evolutionary diversification a carabid beetle lineage, we studied intraspecific and trans-species polymorphisms in mitochondrial NADH dehydrogenase subunit 5 (ND5) gene sequence (1083 bp) four species subgenus Ohomopterus (genus Carabus) central eastern Honshu, Japan. Of species, C. insulicola is parapatric with other three, can hybridize naturally at least two. This possesses two haplotypes remote lineages. We classified ND5 using polymerase chain reaction-restriction fragment length polymorphism TaqI endonuclease for 524 specimens, sequenced 143 samples. Analysis revealed that each was polyphyletic its DNA phylogeny, representing marked case polymorphism. Recent one-way introgression mitochondria from arrowianus nakamurai insulicola, esakii, inferred frequency identical sequences between these direct evidence their contact zones. Other may be due undetected (e.g. maiyasanus) or stochastic lineage sorting ancestral polymorphisms. group has genital lock-and-key system, species-specific subspecies-specific morphology act as barrier hybridization. However, our results demonstrate occurred multiple times, mitochondria, despite differences among, stability within, morphological characters distinguish local populations. Thus, could have been key processes Ohomopterus.