Excision from tRNA genes of a large chromosomal region, carrying avrPphB, associated with race change in the bean pathogen, Pseudomonas syringae pv. phaseolicola

• DOI: 10.1046/j.1365-2958.2000.02133.x
• Publication Date: 2003-03-12T11:34:19Z
• Authors: Robert W. Jackson; John W. Mansfield; Dawn L. Arnold; Ane Sesma; Christopher D. Pa...; Jesús Murillo; John D. Taylor; Alan Vivian
• Keywords: Pseudomonas syringae; Pseudogene

Pseudomonas syringae pv. phaseolicola ( Pph ) race 4 strain 1302A carries avirulence gene avrPphB . Strain RJ3, a sectoral variant from culture, exhibited an extended host range in cultivars of bean and soybean resulting the absence RJ3 chromosome. Complementation with restored phenotype. Both strains showed similar planta growth susceptible cultivars. Analysis indicated loss > 40 kb DNA surrounding Collinearity two genomes was determined for left right junctions deleted region; junction is ≈ 19 20 1302A. Sequencing revealed that region containing inserted into tRNA LYS gene, which re‐formed at A putative lysine pseudogene (ΨtRNA found insertion. All genes were identical orientation Genes near predicted protein homologies products associated virulence locus periodontal pathogen Actinobacillus actinomycetemcomitans Specific oligonucleotide primers differentiate designed used to demonstrate located different regions chromosome other Deletion large represents new route change