The segregation of the Escherichia coli origin and terminus of replication

• DOI: 10.1046/j.1365-2958.2002.03234.x
• Publication Date: 2003-03-12T11:46:18Z
• Authors: Yongfang Li; Kirill Sergueev; Stuart Austin
• Keywords: SeqA protein domain

Escherichia coli chromosome replication forks are tethered to the cell centre. Two opposing models describe how chromosomes segregate. In extrusion-capture model, newly replicated DNA is fed bi-directionally from toward poles, forming new in each half. Starting with origins, chromosomal regions segregate away their sisters progressively as they replicated. The termini last. sister cohesion produces that paired along much of length. origins and most other remain until late cycle, all together. We use a combination microscopy flow cytometry determine relationship origin terminus segregation cycle. Origin frequently follows closely after initiation, strong support model. spatial disposition sequences also fits this Terminus occurs extremely cycle daughter cells separate. As septum begins invaginate, completed transiently held apart at centre, on opposite sides cell. This may facilitate resolution topological linkages between chromosomes.