The use of chlorophyll fluorescence excitation spectra for the non‐destructive in situ assessment of UV‐absorbing compounds in leaves

• DOI: 10.1046/j.1365-3040.2002.00942.x
• Publication Date: 2003-03-12T16:26:02Z
• Authors: Z. G. CEROVIC; A. OUNIS; A. CARTELAT; G. LATOUCHE; Y. GOULAS; S. MEYER; I. MOYA
• Keywords: Epidermis (zoology); Excitation wavelength

Abstract In this study a method was designed to assess non‐destructively the type of UV‐screening compounds present in leaf epidermis. The is based on recording and calculation ratio UV‐excitation spectra chlorophyll fluorescence (FER) from adaxial abaxial sides bifacial leaves, or older younger segments monocotyledonous leaves. logarithm (logFER) matched absorption spectrum UV‐absorbers leaf, as confirmed by its overlap with methanolic extract isolated By using logFER approach, it possible demonstrate that concentration but not classes epidermis are responsible for affected side age contrast, measurements leaves seven dicots one monocot indicated large difference these between species. Finally, shown UV independent emission wavelength, can be used quantitative measurements. This expands spectral domain use ChlF estimation epidermal transmittance.