Disruption of the Arabidopsis photosystem I gene psaE1 affects photosynthesis and impairs growth

• DOI: 10.1046/j.1365-313x.2000.00717.x
• Publication Date: 2003-03-12T22:00:18Z
• Authors: Claudio Varotto; Paolo Pesaresi; Jörg Meurer; Ralf Oelmüller; Sabine Steiner‐Lange; Francesco Salamini; Dario Leister
• Keywords: Photoinhibition; Wild type

Summary The psae1‐1 mutant of Arabidopsis was identified on the basis a decrease in effective quantum yield photosystem II, among collection plants subjected to transposon tagging with Enhancer element. steady‐state redox level and rate re‐oxidation P700 are significantly altered mutants. responsible mutation localised psaE1 , one two genes that encode subunit E I. An additional allele, psae1‐2 by reverse genetics. In wild‐type plants, transcript is expressed at higher than psaE2 mRNA. mutants, however, E1 barely detectable, only small groups cells resulting from somatic reversions. As consequence, amount PsaE protein present reduced. Concomitantly, levels other stromal I subunits (PsaC PsaD) also affected. Mutant showed marked increase light sensitivity photoinhibition. Additional effects psae1 include green pigmentation, an chlorophyll fluorescence approximately 50% growth under greenhouse conditions.