Structural diversity and transcription of class III peroxidases from Arabidopsis thaliana

• DOI: 10.1046/j.1432-1033.2002.03311.x
• Publication Date: 2003-03-11T18:37:33Z
• Authors: Karen G. Welinder; Annemarie F. Just...; Inger V. H. Kjærs...; Rikke B. Jensen; Søren K. Rasmussen; Hans M. Jespersen; Laurent Duroux
• Keywords: 2. Zero hunger; Expressed Sequence Tags; Models, Molecular; 0303 health sciences; DNA, Complementary; Sequence Homology, Amino Acid; Transcription, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Molecular Sequence Data; Arabidopsis; Heme; Introns; Protein Structure, Tertiary; 03 medical and health sciences; Peroxidases; Amino Acid Sequence; Bioteknologi; Genome, Plant; Horseradish Peroxidase

Understanding peroxidase function in plants is complicated by the lack of substrate specificity, high number genes, their diversity structure and our limited knowledge gene transcription translation. In present study we sequenced expressed sequence tags (ESTs) encoding novel heme‐containing class III peroxidases from Arabidopsis thaliana annotated 73 full‐length genes identified genome. total, transcripts 58 these have now been observed. The expression individual was assessed organ‐specific EST libraries compared to 33 which analyzed whole 3, 6, 15, 35 59 days after sowing. Expression root, rosette leaf, stem, cauline flower bud cell culture tissues using gene‐specific highly sensitive reverse transcriptase‐polymerase chain reaction (RT‐PCR). We predicted that 71 could yield stable proteins folded similarly horseradish (HRP). putative mature derived showed 28–94% amino acid identity were all targeted endoplasmic reticulum N‐terminal signal peptides. 20 peptides followed various extensions unknown are not HRP. Ten a C‐terminal extension indicating vacuolar targeting. found majority root. accounted for an impressive 2.2% root ESTs. Rather few organ specificity. Most importantly, constitutively organs with preference represented structurally diverse (< 70% identity). Furthermore, appearing tandem distinct profiles. alignment sequences provides easy access identification orthologous other plant species will provide common platform combining relationships obtained species.