Cloning, characterization and expression analysis of interleukin‐10 from the common carp, Cyprinus carpio L.

• DOI: 10.1046/j.1432-1033.2003.03854.x
• Publication Date: 2003-11-14T00:03:36Z
• Authors: Ram Savan; Daisuke Igawa; Masahiro Sakai
• Keywords: Common carp; Grass carp

Interleukin (IL)-10 was cloned from the common carp (Cyprinus carpio L.) using IL-10 primers head kidney following stimulation with concanavalin A and lipopolysaccharide. The cDNA consisted of a 1096 bp sequence containing 55 5' untranslated region 498 3' region. An open reading frame 543 encoded putative 180 amino acid protein signal peptide 22 acids. signature motif is conserved in sequence. 2083 genomic found to contain five exons interrupted by four introns. With exception much more compact introns, structure similar that mammalian IL-10. By homology, phylogeny analyses, gene designated as Carp expressed head, kidney, liver, spleen intestine during resting phase. also liver vitro