Pig MAP/ITIH4 and haptoglobin are interleukin‐6‐dependent acute‐phase plasma proteins in porcine primary cultured hepatocytes

• DOI: 10.1046/j.1432-1327.2000.01195.x
• Publication Date: 2003-03-11T18:04:33Z
• Authors: Nieves González‐R...; Kasper Hoebe; María A. Alava; Leo van Leengoed; Matilde Piñeiro; Silvana Carmona; María Iturralde; Fermín Lampreave; Andrés Piñeiro
• Keywords: Haptoglobin; Interleukin 1β

The acute‐phase expression of pig MAP (major protein)/ITIH4 (inter‐α‐trypsin inhibitor heavy chain 4) and haptoglobin were analysed in primary cultures isolated hepatocytes response to recombinant human (rh) cytokines: tumor necrosis factor‐α (TNF‐α), interleukin‐1 (IL‐1), interleukin‐6 (IL‐6), as well bacterial lipopolysaccharide (LPS). Analysis MAP/ITIH4 mRNAs was carried out by RT‐PCR amplification. Secreted proteins from the cytokine‐treated quantified immunochemical techniques. Time‐course dose–response experiments show that belong type II proteins, they are specifically induced rhIL‐6 not rhTNF‐α or rhIL‐1. Stimulation cultured with for 48 h at doses 1000 U·mL −1 showed a fourfold fivefold increase concentration medium, while only increased twofold. A similar also observed media LPS‐treated simultaneous generation IL‐6 Kupfer cells present cultures. Albumin secretion decreased after stimulation 100 rhTNF‐α, rhIL‐1 rhIL‐6. Therefore, it can be concluded behaves major protein produced porcine under effect inflammatory cytokines.