Identification of a novel nuclear factor‐kappaB sequence involved in expression of urokinase‐type plasminogen activator receptor

• DOI: 10.1046/j.1432-1327.2000.01350.x
• Publication Date: 2003-03-11T17:45:35Z
• Authors: Yao Wang; Jinjun Dang; Heng Wang; Heike Allgayer; George A. C. Murrell; Douglas Boyd
• Keywords: Sequence motif; Transcription

We have previously defined the promoter of human urokinase‐type plasminogen activator receptor (uPAR) gene in a 188‐bp fragment between bases −141 and +47 relative to translation start site. Here, we report that novel nuclear factor‐kappaB (NF‐κB)‐like sequence (5′‐GGGAGGAGTC‐3′) at −45 is located uPAR one two DNase I‐protected regions, region I −51 −30. This NF‐κB‐like motif differs positions 7–9 from decameric consensus sequences NF‐κB (5′‐GGGRNNYYCC‐3′ where R indicates A or G, Y C T, N any nucleotide) 1 κB‐like motifs (5′‐HGGARNYYCC‐3′ H A, C, nucleotide). Nuclear extracts HCT116 cells contain proteins specifically bind site position −45. Mutation decreased binding transcription factor reduced activity comparison with wild‐type sequences. Co‐transfection dominant negative I‐κB kinase‐2 expression vector by 65–75%. These results demonstrate uncharacterized required for activity.