Signal transduction pathways triggered by fibroblast growth factor receptor 1 expressed in Xenopus laevis oocytes after fibroblast growth factor 1 addition

• DOI: 10.1046/j.1432-1327.2000.01710.x
• Publication Date: 2003-03-11T17:56:57Z
• Authors: Edith Browaeys‐Poly; Katia Cailliau; Jean‐Pierre Vilain
• Keywords: Transduction (biophysics)

Xenopus oocytes expressing fibroblast growth factor receptor 1 (FGFR1) were used as a biological model system to analyse the signal transduction pathways that are triggered by (FGF1). Germinal vesicle breakdown (GVBD) and phosphorylation of extracellular signal‐regulated protein kinase 2 (ERK2) occured 15 h after FGF1 addition. These events Ras‐dependent they blocked Ras dominant negative form. The activity was promoted three upstream effectors, factor‐bound (Grb2), phosphatidylinositol 3‐kinase (PI3K) Src cytoplasmic kinase. activation inhibited Grb2 form (P49L), PI3K inhibitors, including wortmannin, LY294002, N‐SH2 domain p85α SH2 Src. induced PP2, specific inhibitor adaptor recruited homology 2/α‐collagen‐related (Shc) effector, SH2‐Shc prevented GVBD ERK2 FGF1. importance another signalling pathway involving phospholipase Cγ (PLCγ) also investigated. use PLCγ inhibitory peptide, neomycin calcium chelator BAPTA‐AM on FGFR1 or stimulation PDGF‐BB PDGFR‐FGFR1 mutated binding site, phosphorylation. This study shows cascade FGFR1–FGF1 interaction in represents sum PLCγ‐dependent pathways. It emphasizes role played their connections with transduction.