Endothelium‐dependent Relaxation of Canine Uterine Artery in Response to Acetylcholine: the Possible Involvement of Alternative Pathways

• DOI: 10.1046/j.1439-0442.2003.00568.x
• Publication Date: 2003-11-21T03:58:34Z
• Authors: S. Pešić; L. Grbović; A. Jovanović; M. Radenković; D. Stojić; Z. Cvetković; I. Ilić
• Keywords: Endothelium-derived hyperpolarizing factor

Summary The effect of acetylcholine on the isolated, pre‐contracted, uterine artery non‐pregnant dog was investigated. Acetylcholine‐induced concentration‐dependent relaxation isolated canine with endothelium (pEC 50 = 6.48 ±0.01, n 37) and without arterial segments denuded endothelium. Indomethacin, 4‐aminopyridine (10 −5 m ) pre‐contraction K + ‐rich Krebs–Ringer bicarbonate solution had no acetylcholine‐induced relaxation. N G ‐nitro‐ l ‐arginine ( ‐NOARG) inhibited evoked by acetylcholine. Indomethacin applied ‐NOARG led to further inhibition In presence both indomethacin, 4‐aminopiridine did not provoke artery. It is concluded that probably mediated endothelial production nitric oxide (NO). However, if NO‐synthase inhibited, vasorelaxation may be, in part, through activation cyclooxygenase pathway.