HAPPY mapping in a plant genome: reconstruction and analysis of a high‐resolution physical map of a 1.9 Mbp region ofArabidopsis thalianachromosome 4

• DOI: 10.1046/j.1467-7652.2003.00001.x
• Publication Date: 2003-03-12T16:13:50Z
• Authors: Madan Thangavelu; Allan B. James; Alan Bankier; Glenn J. Bryan; Paul H. Dear; Robbie Waugh
• Keywords: Physical mapping

Summary HAPPY mapping is an in vitro approach for defining the order and spacing of DNA markers directly on native genomic DNA. This cloning‐free technique based analysing segregation amplified from high molecular weight which has been broken randomly ‘segregated’ by limiting dilution into subhaploid samples. It a uniquely versatile tool, allowing construction genome maps with flexible ranges resolutions. Moreover, it applicable to plant genomes, many techniques pioneered animal genomes are inapplicable or inappropriate. We report here its demonstration reconstructing physical map 1.9 Mbp region around FCA locus Arabidopsis thaliana. The resulting map, spanning 10% chromosome 4, excellent agreement sequence mean marker 16 kbp. argue that any required resolution can be made immediately relatively little effort most species and, furthermore, such greatly aid regional genome‐wide maps.