Reactions of paratracheal cells of resistant and susceptible carnation (Dianthus caryophyllus) cultivars to vascular invasion by Fusarium oxysporum f. sp. dianthi

• DOI: 10.1046/j.1469-8137.2002.00499.x
• Publication Date: 2003-03-12T15:38:27Z
• Authors: G. B. Ouellette; R. P. Baayen; M. Simard; D. Rioux
• Keywords: Dianthus; Vascular tissue; Suberin; Pectin

Summary Here, cell and wall modifications in resistant (cv. I ) susceptible II carnation inoculated with Fusarium oxysporum are characterized. Samples, of various days after inoculation (dai), were double‐fixed gold‐complexed probes used for cellulose, pectin, chitin. In cv. , mature paratracheal cells contained appositions mostly bound by a cellulose layer over the densely opaque native walls. Middle lamellae younger cells, many hyperplastic much distorted, obliterated, displayed mingling cellulose‐labelled ‐unlabelled components; adjoining inner suberin layers. Cell limited, but early pathogen invasion was restricted to middle despite pronounced host content alterations. both cvs, microfilaments linked often small, thin‐walled fungal vessels turn coating on their walls, extended into seemingly associated reactions, excreted as bodies vacuoles. The present results highlight that extensive reactions modifications, including deposition tissue, main initial factors defence conducive final confinement.