Developmental Changes in the Differential Expression of Two Serotonin 5‐HT3 Receptor Splice Variants in the Rat

• DOI: 10.1046/j.1471-4159.1995.65020475.x
• Publication Date: 2010-07-15T21:35:14Z
• Authors: M.‐C. Miquel; M. B. Emerit; J. A. Gingrich; A. Nosjean; M. Hamon; S. El Mestikawy
• Keywords: GABBR1

Abstract: PCR was used to isolate identical partial cDNA clones encoding a serotonin 5‐HT 3 receptor subunit from rat nodose and superior cervical ganglia. The amino acid sequence predicted these clones, extending the putative transmembrane domain I stop codon, demonstrated 93% homology with A (R‐A) cloned NCB 20 hybridoma mouse neuroblastoma/Chinese hamster embryonic brain cells. Comparison of sequences gene this revealed five deletion, GSLLP, located within second intracellular loop subunit. This deletion shown occur at an intron/exon junction. Therefore, alternative splicing probably responsible for presence short (5‐HT R‐A S ) long L forms mRNA in experiments, specific primers upstream downstream GSLLP were detect reverse transcribed mRNAs. fragment (92 bp), corresponding deleted form, (107 nondeleted amplified various regions CNS peripheral ganglia rat, as well NG108‐15 In adult ratio two varied very little one tissue another, form only ∼10% total mRNA. Study their respective distributions during ontogeny differential expression some tissues late development, day 17 (E17) or E20. particular, amounted about one‐third cerebral cortex hippocampus E17, proportion reached 50 75% ganglion ganglion, respectively, These data indicate that is regulated PNS development rat.