Expression of T‐Cadherin (CDH13, H‐Cadherin) in Human Brain and Its Characteristics as a Negative Growth Regulator of Epidermal Growth Factor in Neuroblastoma Cells

• DOI: 10.1046/j.1471-4159.2000.0741489.x
• Publication Date: 2003-03-12T06:44:22Z
• Authors: Tamotsu Takeuchi; Akiko Misaki; Sheng‐Ben Liang; Akiko Tachibana; Nakanobu Hayashi; Hiroshi Sonobe; Yuji Ohtsuki
• Keywords: Adult; Brain Chemistry; 0301 basic medicine; DNA, Complementary; Epidermal Growth Factor; Sequence Homology, Amino Acid; Molecular Sequence Data; Antibodies, Monoclonal; Blotting, Northern; Cadherins; Transfection; Gene Expression Regulation, Neoplastic; Neuroblastoma; 03 medical and health sciences; Tumor Cells, Cultured; Humans; RNA, Messenger; Cloning, Molecular; Cell Division; In Situ Hybridization

Abstract: In the present study, we first examined expression of T‐cadherin in human CNS by northern blot analysis, immunohistochemical staining, and situ hybridization. Northern analysis demonstrated adult cerebral cortex, medulla, thalamus, midbrain. Immunohistochemical staining with a newly generated monoclonal antibody, designated MA‐511, revealed strong neural cell surface membrane neurites medulla oblongata, nucleus olivaris. Little or no was found spinal cord. We further various developing nervous systems, that lower brain than brain. hybridization cells oblongata olivaris, but not cord, possessed molecules. transfected T‐cadherin‐negative TGW NH‐12 neuroblastoma cDNA‐containing vector. T‐cadherin‐expressing lost mitogenic proliferative response to epidermal growth factor. Epidermal factor is known be required for proliferation stem cells. This finding, together those suggests functions as negative regulator growth.