The Transcription Factor E2F1 Modulates Apoptosis of Neurons

• DOI: 10.1046/j.1471-4159.2000.0750091.x
• Publication Date: 2003-03-12T06:46:09Z
• Authors: Sheng T. Hou; Debbie Callaghan; Marie‐Christine F...; Irene Hill; Liping Kang; Bernard Massie; Paul Morley; Christine Murray; Ingrid Rasquinha; Ruth Slack; John P. MacManus
• Keywords: E2F1; Staurosporine; Fragmentation

Abstract : The transcription factor E2F1 is known to mediate apoptosis in isolated quiescent and postmitotic cardiac myocytes, its absence decreases the size of brain infarction following cerebral ischemia. To demonstrate directly that modulates neuronal apoptosis, we used cultured cortical neurons show a temporal association expression with increased apoptosis. Cortical lacking (derived from ‐/‐ mice) were resistant staurosporine‐induced as evidenced by significantly lower caspase 3‐like activity lesser number cells apoptotic morphology comparison cultures derived wild‐type mice. Furthermore, overexpressing alone using replication‐deficient recombinant adenovirus was sufficient cause cell death appearance hallmarks such threefold increase laddered DNA fragmentation, situ endlabeled numbers punctate nuclei. Taken together, conclude plays key role modulating