Role of capacitative calcium entry on glutamate‐induced calcium influx in type‐I rat cortical astrocytes

• DOI: 10.1046/j.1471-4159.2001.00539.x
• Publication Date: 2003-03-12T06:56:45Z
• Authors: Paola Pizzo; Andrea Burgo; Tullio Pozzan; Cristina Fasolato
• Keywords: Calcium in biology; Neuroglia; Calcium Signaling

Capacitative calcium entry (CCE) has been described in a variety of cell types. To date, little is known about its role the CNS, and particular cross‐talk between glia neurons. We have first analyzed properties CCE astrocytes culture, comparison with that rat basophilic leukemia line (RBL‐2H3), model where release‐activated Ca 2+ (CRAC) channels unambiguously correlated CCE. here show (i) activated by store depletion influx induced glutamate share same pharmacological profile RBL‐2H3 cells (ii) glutamate‐induced plays primary glutamate‐dependent intracellular concentration ([Ca ] i ) oscillations, being these latter reduced frequency amplitude micromolar concentrations La 3+ . Finally, we compared expression various mammalian transient receptor potential genes ( TRP cells. Despite similar cells, pattern very different. The involvement s dependent activation discussed.