Anandamide, but not 2‐arachidonoylglycerol, accumulates during in vivo neurodegeneration

• DOI: 10.1046/j.1471-4159.2001.00542.x
• Publication Date: 2003-03-12T06:56:36Z
• Authors: Henrik H. Hansen; Patricia C. Schmid; Petra Bittigau; Isabel Lastres‐Be...; Fernando Berrendero; Jorge Manzanares; Chrysanthy Ikonom...; Harald H. O. Schmid; J. Javier Fernánd...; Harald S. Hansen
• Keywords: Cerebral Cortex; Male; 0301 basic medicine; N-Methylaspartate; Polyunsaturated Alkamides; Arachidonic Acids; Corpus Striatum; Glycerides; Rats; Rats, Sprague-Dawley; 03 medical and health sciences; Ethanolamines; Cannabinoid Receptor Modulators; Nerve Degeneration; Excitatory Amino Acid Agonists; Animals; Craniocerebral Trauma; RNA, Messenger; Dizocilpine Maleate; Excitatory Amino Acid Antagonists; Brain Concussion; Endocannabinoids

Endogenous cannabinoid receptor ligands (endocannabinoids) may rescue neurons from glutamate excitotoxicity. As these substances also accumulate in cultured immature neurons following neuronal damage, elevated endocannabinoid concentrations may be interpreted as a putative neuroprotective response. However, it is not known how glutamatergic insults affect in vivo endocannabinoid homeostasis, i.e. N‐arachidonoylethanolamine (anandamide) and 2‐arachidonoylglycerol (2‐AG), as well as other constituents of their lipid families, N‐acylethanolamines (NAEs) and 2‐monoacylglycerols (2‐MAGs), respectively. Here we employed three in vivo neonatal rat models characterized by widespread neurodegeneration as a consequence of altered glutamatergic neurotransmission and assessed changes in endocannabinoid homeostasis. A 46‐fold increase of cortical NAE concentrations (anandamide, 13‐fold) was noted 24 h after intracerebral NMDA injection, while less severe insults triggered by mild concussive head trauma or NMDA receptor blockade produced a less pronounced NAE accumulation. By contrast, levels of 2‐AG and other 2‐MAGs were virtually unaffected by the insults employed, rendering it likely that key enzymes in biosynthetic pathways of the two different endocannabinoid structures are not equally associated to intracellular events that cause neuronal damage in vivo. Analysis of cannabinoid CB1 receptor mRNA expression and binding capacity revealed that cortical subfields exhibited an up‐regulation of these parameters following mild concussive head trauma and exposure to NMDA receptor blockade. This may suggest that mild to moderate brain injury may trigger elevated endocannabinoid activity via concomitant increase of anandamide levels, but not 2‐AG, and CB1 receptor density.