Inhibition of tumor necrosis factor‐α attenuates wound breaking strength in rats

• DOI: 10.1046/j.1524-475x.2000.00547.x
• Publication Date: 2003-03-12T23:54:08Z
• Authors: Richard H Lee; David T Efron; Udaya Tantry; Christina Stuelten; Lyle L Moldawer; Amer Abouhamze; Adrian Barbul
• Keywords: Intraperitoneal injection; Subcutaneous injection

Exogenous administration of tumor necrosis factor‐α has been shown to both enhance and attenuate cutaneous healing in a dose‐dependent manner. We examined the effects factor inhibition wound by systemic local factor‐binding protein. Male Balb/C mice underwent dorsal skin incision with subcutaneous implantation 20 mg polyvinyl alcohol sponges (4 per animal). In Experiment I, one group ( n = 20) received intraperitoneal injections protein (3 mg/kg) at time wounding, while another saline. Four animals from each were euthanized on days 1, 3, 5, 7, 14 postwounding. II, 10) an injection wounding every third day thereafter. Another saline III, single 7), or postwounding 4 7 7). respectively). All Experiments II III killed 14. Wound breaking strengths assessed using tensiometer. fluid collected implanted was assayed for levels biological assay enzyme‐linked immunosorbent assay, respectively. Collagen gene expression sponge granulomata Northern analysis. deposition quantified measuring hydroxyproline content. Wounds significantly weaker that repeated mean strength 93.1 g vs. 186.6 controls p < 0.05). groups either 0, 4, no different than their respective controls. There difference content between any analysis collagen I also revealed differences. These data indicate continued resulted wounds corresponding differences content, expression. This suggests throughout leads qualitatively impaired without quantitative alteration deposition.