Acceleration of wound contraction and healing with a photocrosslinkable chitosan hydrogel

• DOI: 10.1046/j.1524-475x.2001.00513.x
• Publication Date: 2003-03-12T23:54:42Z
• Authors: MASAYUKI ISHIHARA; KATSUAKI ONO; MASATO SATO; KUNIAKI NAKANISHI; YOSHIO SAITO; HIROFUMI YURA; TAKEMI MATSUI; HIDEMI HATTORI; MASANORI FUJITA; MAKOTO KIKUCHI; AKIRA KURITA
• Keywords: Ultraviolet light

Application of ultraviolet light irradiation to a photocrosslinkable chitosan aqueous solution resulted in an insoluble, flexible hydrogel like soft rubber within 60 seconds. In order evaluate its accelerating effect on wound healing, full‐thickness skin incisions were made the backs mice and subsequently was added into irradiated with UV for 90 significantly induced contraction accelerated closure healing compared untreated controls. Histological examination also showed advanced rate first 2 days tissue fill 4 hydrogel‐treated wounds. Furthermore, cell culture studies, medium supplemented 5% fetal–bovine serum found be chemoattractant human dermal fibroblasts invasion chamber assay using filters coated Matrigel migration assay. Due ability accelerate may become accepted as occlusive dressing management.