Transperitoneal Transport of Glucose In Vitro

• DOI: 10.1046/j.1525-1594.2000.06637.x
• Publication Date: 2003-03-11T03:10:12Z
• Authors: Krystyna Czyżewska; Beata Szary; Jacek Waniewski
• Keywords: Analysis of Variance; Detergents; Models, Biological; Epithelium; Icodextrin; Permeability; Anti-Bacterial Agents; Diffusion; 03 medical and health sciences; Glucose; 0302 clinical medicine; Dialysis Solutions; Linear Models; Animals; Diffusion Chambers, Culture; Rabbits; Gentamicins; Peritoneum; Glucans; Algorithms; Deoxycholic Acid

Abstract: The effect of fluid mixing intensification, damage of mesothelial cells, gentamicin, and icodextrin on the diffusive glucose transport across the peritoneal membrane were evaluated in in vitro studies. A mathematical model of mass transport was used to calculate the diffusive permeability, expressed as a diffusive permeability coefficient (P). In the control conditions, the rate of glucose transfer from the interstitial to the mesothelial side of membrane (I→M) and in the opposite direction (M→I) remained constant, and the P value at mean was 2,731 ± 1,493 × 10−4 (cm × s−1). The change of the stirring rate from 5.5 to 11 ml/min increased P values by about 74% for transport direction I→M and 58% for M→I, and the change from 11 to 22 ml/min enhanced P at mean by about 42% for both directions. The damage of the mesothelial layer, using sodium deoxycholate (2.5 mmol/L; 103.6 mg%), increased the glucose transfer from the interstitial to the mesothelial side of the peritoneum by 41% and to the opposite direction by 70%. Addition of icodextrin to the glucose solution increased glucose bidirectional transport at mean by about 14% for I→M and 24% for M→I. Furthermore, gentamicin did not change the I→M transfer, but diminished M→I transport by about 12%. In conclusion, the reduction of unstirred fluid layers at the mesothelium and the interstitium‐fluid interfaces, removal of mesothelium, and addition of icodextrin increased the diffusive glucose transport in vitro; unstirred fluid layers restricted glucose transfer (I→M) more than the mesothelium; and peritoneal glucose transport, directed from the mesothelial to the interstitial side of the peritoneum, decreased slightly after the addition of gentamicin.