Bacterial Profile of Middle Ear Fluid with Recurrent Acute Otitis Media Infection Using Culture Independent 16S rDNA Gene Sequencing

• DOI: 10.1055/s-0038-1675786
• Publication Date: 2018-12-11T23:59:41Z
• Authors: Anurag Girdhar; Jagdish Chinnappa; Feroze Ganaie; Vandana Govindan; Kadahalli Ravikumar; Geetha Nagaraj
• Keywords: Microbiological culture; Streptococcus mitis; Neisseria

Background Recurrent otitis media is one of the common infections childhood. The causative bacterial pathogen major risk factors recurrent infection. With limited availability Indian data, we performed this study to identify pathogens. Materials and Methods Otitis cases were diagnosed based on clinical criteria. Thirty-six middle ear fluid (MEF) samples collected by tympanocentesis cultured for Seventy-eight per cent had three previous episodes in past 6 months; remaining 22% four preceding months. At time sample collection, all patients antibiotic coverage. Genomic DNA was extracted from MEF using Qiagen mini Kit. 16s rDNA polymerase chain reaction (PCR) quantitative multiplex (qmPCR) Streptococcus pneumoniae these samples. pneumoniae–positive serotyped PCRSeqTyping. Results None 36 showed growth conventional culture. PCR identified pathogens 33 Four gave mixed reads. organisms Neisseria spp. other than meningitidis (n = 7), N. 8), Lactococcus 5), S. 2), Pseudomonas aeruginosa Haemophilus influenzae 1), Salmonella infantis Staphylococcus epidermidis auricularis sp. 1). qmPCR detected presence six PCRSeqTyping able Serotype 19A two positive pneumoniae. Conclusion demonstrates usefulness protocol a culture-independent method. predominant species followed Detection pneumococci correlated well with qmPCR-based detection