Effect of the mutation (C3435T) at exon 26 of the MDR1 gene on expression level of MDR1 messenger ribonucleic acid in duodenal enterocytes of healthy Japanese subjects

• DOI: 10.1067/mcp.2002.122055
• Publication Date: 2002-08-24T22:14:26Z
• Authors: Tsutomu Nakamura; Toshiyuki Sakaeda; Masanori Horinouchi; Takao Tamura; Nobuo Aoyama; Toshiro Shirakawa; Masafumi Matsuo; Masato Kasuga; Katsuhiko Okumura
• Keywords: Heterozygote advantage

The effect of the C3435T mutation at exon 26 MDR1 gene on expression levels messenger ribonucleic acid (mRNA) was evaluated by means real-time polymerase chain reaction in 51 biopsy specimens duodenum obtained from 13 healthy Japanese subjects. mRNA were 0.38 +/- 0.15, 0.56 0.14, and 1.13 0.42 (mean value SE) subjects with homozygote wild-type allele (C/C), compound heterozygote mutant T (C/T), (T/T), respectively, reasonably explaining lower digoxin serum concentration after administration a single oral dose to harboring allele. Good correlation (r =.797; P <.01) observed between concentrations CYP3A4 individual specimens. This finding suggested plasma substrates for gene.