Comparison of meat quality, muscle-fibre characteristics and the Sirt1/AMPK/PGC-1α pathway in different breeds of pigs

• DOI: 10.1071/an23275
• Publication Date: 2024-07-07T23:56:50Z
• Authors: Yi Gao; Zhaohua Li; Qi Zhang; Tong Hao; Hongliang Liu; Qingyu Liu; Lizhai Liu; Zhibin Zhang; Yongsheng Yu; Na Li
• Keywords: Muscle fibre; Beef Cattle

Context Muscle fibre characteristics are important internal factors that can directly affect pork quality. Especially muscle-fibre types interconvert with the influence of certain factors. Aims The purpose this experiment was to study differences in meat quality among Songliao black pigs, Jilin Hua pigs and Dongliao molecular mechanism differences. Methods First, conventional meat-quality traits each breed were determined. Second, different muscles analysed by haematoxylin–eosin staining immunohistochemistry. Next, activity several key oxidative/glycolytic enzymes detected kits. Finally, quantitative polymerase chain reaction used analyse expression abundance myosin heavy-chain (MyHC) genes related muscle fibre-type transformation. Key results measurement showed superior water-holding capacity, tenderness intra-muscular fat; capacity. diameter area fibres smallest, density largest, proportion oxidative fibres, enzyme highest followed pigs. proportions ranged from 10.37% 33.6% 6.96% 26.42% 5.86% 17.42% psoas major exhibited smallest diameter, density, triceps brachii, biceps femoris longissimus thoracis. brachii significantly greater than those abundances AMP-activated protein kinase (AMPK), silencing information regulator 1 (SIRT1) peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) (Sirt1/AMPK/PGC-1α) pathway mitochondrial function-related Conclusions had best quality, This suggests transformation be promoted through Sirt1/AMPK/PGC-1α pathway. Implications By analysing differences, it provide data reference direction for further improvement three