Transcriptional response of the bovine endometrium and embryo to endometrial polymorphonuclear neutrophil infiltration as an indicator of subclinical inflammation of the uterine environment

• DOI: 10.1071/rd11171
• Publication Date: 2012-01-27T05:35:03Z
• Authors: Michael Hoelker; Dessie Salilew-Wo...; Marc Drillich; Grosse-Brinkhaus ...; Nasser Ghanem; Leopold Goetze; Dawit Tesfaye; Karl Schellander; Wolfgang Heuwieser
• Keywords: Endometritis; Subclinical infection

The aim of the present study was to analyse effect subclinical endometritis on endometrial and embryonic gene expression. A total 49 cows at either Day 0 or 7 oestrous cycle (62-83 days post partum) following superovulation were classified as having (SE-0, SE-7) a healthy endometrium (HE-0, HE-7) basis cytological evaluation. Endometrial samples associated embryos subjected global transcriptome analysis using Bovine GeneChip (Affymetrix, Santa Clara, CA, USA) aberrant transcript profiles observed in SE-0 SE-7 cows. At 0, 10 transcripts found be differentially expressed samples. Specifically, PDZK1, PXDN, DDHD2, GPLD1 SULT1B1 genes downregulated, whereas PKIB, LOC534256, BT29392, LYZ S100A14 upregulated Similarly, 11 regulated 7. Of these, GNPTG, BOLA-DQA5, CHD2, LOC541226, VCAM1 ARHGEF2 PSTPIP2, BT236441 MGC166084 Accordingly, health status affected number flushed, transferable embryos. In all, 20 blastocysts derived from HE-7 GZMK, TCEAL4, MYL7, ADD3 THEM50B upregulated, NUDCD2, MYO1E, BZW1, EHD4 GZMB downregulated. conclusion, polymorphonuclear neutrophil infiltration an indicator is with changes expression patterns, including involved cell adhesion immune modulation. Consequently, affects embryos, related membrane stability, apoptosis.