Soluble Delta-like ligand 1 alters human endometrial epithelial cell adhesive capacity

• DOI: 10.1071/rd15313
• Publication Date: 2015-11-30T03:54:48Z
• Authors: Michelle Van Sind...; Jennifer Oyanedel; Ellen Menkhorst; Carly Cuman; Katarzyna Rainczuk; Amy Winship; Lois Salamonsen; Tracey Edgell; Evdokia Dimitriadis
• Keywords: Immunostaining

The endometrium undergoes substantial morphological and functional changes to become receptive embryo implantation enable establishment of a successful pregnancy. Reduced Delta-like ligand 1 (DLL1, Notch ligand) in the is associated with infertility. DLL1 can be cleaved by 'a disintegrin metalloprotease' (ADAM) proteases produce soluble that may act inhibit signalling. We used an enzyme-linked immunosorbent assay quantify uterine lavages from fertile infertile women secretory phase menstrual cycle. also determined cellular location immunostaining intensity ADAM12 17 human throughout Functional effects receptivity were analysed using vitro adhesion proliferation assays gene expression analysis signalling targets. Soluble was significantly increased lavage samples compared This coincided ADAM17 detected endometrial luminal epithelium mid-secretory women. inhibited adhesive capacity epithelial cells via downregulation helix-loop-helix hairy/enhancer split family member HES1 mRNA. Thus, serve as suitable target or potential biomarker for receptivity.