Hematopoietic contribution to skeletal muscle regeneration by myelomonocytic precursors

• DOI: 10.1073/pnas.0405361101
• Publication Date: 2004-09-08T01:33:01Z
• Authors: Regis Doyonnas; Mark A. LaBarge; Alessandra Sacco; Carol Charlton; Helen M. Blau
• Keywords: Monocyte; Precursor cell

Adult bone marrow-derived cells can participate in muscle regeneration after marrow transplantation. In recent studies a single hematopoietic stem cell (HSC) was shown to give rise that not only reconstituted all of the lineages blood, but also contributed mature fibers. However, relevant HSC derivative with this potential has yet been definitively identified. Here we use fluorescence-activated sorter-based protocols test distinct fractions and show containing c-kit(+) immature myelomonocytic precursors are capable contributing fibers i.m. injection. Although these belong myeloid lineage, they do include CD11b(+) cells, such as macrophages. Of four sources macrophages tested were derived either from monocytic culture, marrow, peripheral blood granulocyte colony-stimulating factor mobilization, or injured muscle, none muscle. addition, transplantation isolated CD11b-Cre-transgenic mice into Cre-reporter strain (Z/EG), no GFP myofibers detected, demonstrating expressing CD11b fuse myofibers. Irrespective underlying mechanisms, data suggest derivatives integrate regenerating exist pool known progenitors.