Structure of the KvAP voltage-dependent K + channel and its dependence on the lipid membrane

• DOI: 10.1073/pnas.0507651102
• Publication Date: 2005-10-14T00:33:29Z
• Authors: Seok-Yong Lee; Alice Lee; Jiayun Chen; Roderick MacKinnon
• Keywords: 0301 basic medicine; Membrane Lipids; 03 medical and health sciences; Potassium Channels, Voltage-Gated; Protein Conformation; Lipid Bilayers; Kv1.2 Potassium Channel; Crystallization

Voltage-dependent ion channels gate open in response to changes cell membrane voltage. This form of gating permits the propagation action potentials. We present two structures voltage-dependent K + channel KvAP, complex with monoclonal Fv fragments (3.9 Å) and without antibody (8 Å). also studied KvAP disulfide cross-bridges lipid membranes. Analyzing these data context crystal structure Kv1.2 EPR on we reach following conclusions: ( i ) is similar a very modest difference orientation its voltage sensor; ii mAb are not source non-native conformations structures; iii because contains separate loosely adherent domains, required maintain their correct relative orientations, iv model consistent proposal sensing through movement an arginine-containing helix-turn-helix element at protein-lipid interface.