Intranasal immunization with heterologously expressed polysaccharide protects against multiple Pseudomonas aeruginosa infections

• DOI: 10.1073/pnas.0608657104
• Publication Date: 2007-03-06T01:44:34Z
• Authors: Antonio DiGiandom...; Jayasimha Rao; Katie Harcher; Tanweer S. Zaidi; Jason Gardner; Alice N. Neely; Gerald B. Pier; Joanna B. Goldberg
• Keywords: Salmonella enterica

Surface-expressed bacterial polysaccharides are often immunodominant, protective antigens. However, these antigens chemically and serologically highly heterogeneous, conjugation to protein carriers is necessary enhance their immunogenicity. Here we show the efficacy of intranasal immunization mice with attenuated Salmonella enterica serovar Typhimurium expressing O antigen portion Pseudomonas aeruginosa lipopolysaccharide. P. an ideal model system because it can cause a myriad localized systemic infections. In particular, this bacterium leading hospital-acquired pneumonia responsible for infections after burns eye injury. addition, there mouse models infection that mimic clinical manifestations Immunized were protected against infection, long-lasting immunity acute pneumonia, whereas immunized containing only cloning vector or PBS not. Prophylactic therapeutic administration sera from vaccinated animals naive mice. Intranasal vaccination also provided complete protection reduced pathology corneal abrasions. These results indicate delivery heterologously expressed polysaccharide provides at distinct sites infection. This approach expression as recombinant immunogens could be easily adapted develop vaccines many infectious agents, without need complicated purification procedures.