Smad-interacting protein-1 (Zfhx1b) acts upstream of Wnt signaling in the mouse hippocampus and controls its formation

• DOI: 10.1073/pnas.0609863104
• Publication Date: 2007-07-21T00:48:49Z
• Authors: Amaya Miquelajaur...; Tom Van de Putte; Alexander Polyakov; Anjana Nityanandam; Sridhar Boppana; Eve Seuntjens; Anton Karabinos; Yujiro Higashi; Danny Huylebroeck; Victor Tarabykin
• Keywords: EXPRESSION; Telencephalon; brain; knockout; hirschsprung-disease; Apoptosis; Hippocampus; mowat-wilson-syndrome; Mice; 03 medical and health sciences; mental retardation syndrome; E-CADHERIN; expression; Animals; BRAIN; gene; Promoter Regions, Genetic; development; Cell Proliferation; Homeodomain Proteins; 0303 health sciences; MUTATIONS; sfrp1; Stem Cells; MOWAT-WILSON-SYNDROME; sip1; JNK Mitogen-Activated Protein Kinases; Gene Expression Regulation, Developmental; Membrane Proteins; HIRSCHSPRUNG-DISEASE; mutations; GENE; Up-Regulation; Repressor Proteins; Wnt Proteins; BINDING-PROTEIN; cortex; Gene Expression Regulation; binding-protein; Mutation; SIP1; Intercellular Signaling Peptides and Proteins; MENTAL RETARDATION SYNDROME; e-cadherin; Gene Deletion; telencephalon; Signal Transduction

Smad-interacting protein-1 (Sip1) [Zinc finger homeobox (Zfhx1b)] is a transcription factor implicated in the genesis of Mowat–Wilson syndrome humans. Sip1 expression dorsal telencephalon mouse embryos was documented from E12.5. We inactivated gene specifically cortical precursors. This resulted lack entire hippocampal formation. mutant mice exhibited death differentiating cells and decreased proliferation region prospective hippocampus dentate gyrus. The Wnt antagonist Sfrp1 ectopically activated, whereas activity noncanonical effector, JNK, down-regulated embryonic mice. In cells, protein detected on promoter both genes showed mutually exclusive pattern suggesting that negatively regulated by Sip1. therefore essential to development gyrus, able modulate signaling these regions.