Dynamic behavior of paired claudin strands within apposing plasma membranes

• DOI: 10.1073/pnas.0630649100
• Publication Date: 2003-04-01T19:25:08Z
• Authors: Hiroyuki Sasaki; Chiyuki Matsui; Kyoko Furuse; Yuko Mimori-Kiyosue; Mikio Furuse; Shoichiro Tsukita
• Keywords: Claudin; Photobleaching

The tight junction (TJ) strand is a linear proteinaceous polymer spanning plasma membranes, and each TJ associates laterally with another in the apposing membranes of adjacent cells to form “paired” strands. Claudins have been identified as major constituents strands, when exogenously expressed L fibroblasts, they polymerize into paired which are morphologically similar strands epithelia. Here, we show that fusion protein GFP claudin-1 can also allowing us directly observe individual claudin live real time. These showed more dynamic behavior than expected; were occasionally broken annealed, dynamically associated other both an end-to-side side-to-side manner. Through this network was reorganized dynamically. Furthermore, fluorescence recovery after photobleaching analyses revealed molecules not mobile within Although these observations necessarily representative per se epithelial cells, provide important information on structural kinetic properties situ significant implications for barrier function TJs.