High-throughput sequencing allows the identification of binding molecules isolated from DNA-encoded chemical libraries

• DOI: 10.1073/pnas.0805130105
• Publication Date: 2008-11-11T02:25:47Z
• Authors: Luca Mannocci; Yixin Zhang; Jörg Scheuermann; Markus Leimbacher; Gianluca De Bellis; Ermanno Rizzi; Christoph Dumelin; Samu Melkko; Dario Neri
• Keywords: Sequencing by ligation

DNA encoding facilitates the construction and screening of large chemical libraries. Here, we describe general strategies for stepwise coupling coding fragments to nascent organic molecules throughout individual reaction steps as well first implementation high-throughput sequencing identification relative quantification library members. The methodology was exemplified in a DNA-encoded containing 4,000 compounds discovery binders streptavidin, matrix metalloproteinase 3, polyclonal human IgG.