Posttranscriptional regulation of interleukin-10 expression by hsa-miR-106a

• DOI: 10.1073/pnas.0808743106
• Publication Date: 2009-03-24T01:30:41Z
• Authors: Amit Sharma; Manish Kumar; Jyotirmoi Aich; Manoj Hariharan; Samir K. Brahmachari; Anurag Agrawal; Balaram Ghosh
• Keywords: EGR1

IL-10 is a key regulator of the immune system that critically determines health and disease. Its expression finely tuned both at transcriptional posttranscriptional levels. Although importance regulation has been previously shown, understanding underlying mechanisms still in its infancy. In this study, using combination bioinformatics molecular approaches, we report microRNA (hsa-miR-106a) regulates expression. The hsa-miR-106a binding site 3' UTR IL10 identified by site-directed mutagenesis studies. Also, involvement transcription factors, Sp1 Egr1, concomitant decrease expression, also demonstrated. summary, our results showed may be regulated miR-106a, which turn transcriptionally Egr1 Sp1.