Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation

• DOI: 10.1073/pnas.0832308100
• Publication Date: 2003-06-10T17:06:36Z
• Authors: Warren R. Zipfel; Rebecca M. Williams; Richard Christie; Alexander Yu Nikitin; Bradley T. Hyman; Watt W. Webb
• Keywords: Two-photon excitation microscopy; Fluorescence-lifetime imaging microscopy

Multicolor nonlinear microscopy of living tissue using two- and three-photon-excited intrinsic fluorescence combined with second harmonic generation by supermolecular structures produces images the resolution detail standard histology without use exogenous stains. Imaging indicators within tissue, such as nicotinamide adenine dinucleotide, retinol, indoleamines, collagen provides crucial information for physiology pathology. The efficient application multiphoton to imaging requires knowledge optical properties specific cell components. Here we compile demonstrate applications involving a range molecules molecular assemblies that enable direct visualization morphology, metabolism, disease states Alzheimer's cancer.